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| Status |
Public on Sep 13, 2017 |
| Title |
Characterization of the small RNAome in acute myeloid leukemia and discovery of novel small RNA species by deep sequencing (Illumina) |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We used small RNA seq (Illumina) to characterise and quantify the miRNA and small non-coding (snc)RNA transcriptome of 38 libraries of size-fractionated RNA obtained from AML patients and normal bone marrow. We detected the expression of 765 known miRNAs and 2554 uniquely located species of sncRNAs. A total of 575 potential miRNA candidate hairpins were identified. We also identified 37 C/D Box and 51 H/ACA Box, potential novel snoRNAs.
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| Overall design |
We sequenced 38 libraries obtained from 34 cytogenetically distinct cases of AML and 1 normal bone marrow from healthy donor. Duplicate experiments, which involved 2 independent RNA size fractionation and library preparation, were performed for 2 patient samples and for the normal bone marrow.
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| Contributor(s) |
Wang J, Marzec J, Manodoro F, Mein C, Curtotti A, Young BD, Debernardi S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 11, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Jacek Dariusz Marzec |
| E-mail(s) |
j.marzec@qmul.ac.uk
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| Organization name |
Barts Cancer Institute
|
| Department |
Centre for Haemato-Oncology
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| Lab |
Cancer Genomics
|
| Street address |
Charterhouse Square
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| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (38)
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| Relations |
| SRA |
SRP007826 |
| BioProject |
PRJNA145991 |
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