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| Status |
Public on Aug 17, 2011 |
| Title |
Deletion of HDAC1 induces caspase-independent autophagic cell death and mitotic defect in Hep3B cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
HDAC1 aberrantly over expressed in HCC and it was considered as an oncogene gene in HCC development. So we want to analyze in greater detail of the genes regulated by HDAC1 using microarray assay.
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| Overall design |
Hep3B RNA was extracted after transfected by HDAC1 shRNA or scrambled shRNA (Mock) for 72hr. For microarray analysis of gene expression unpon depletion of HDAC1, 0.5 μg of total RNA was used to make biotin-labeled cRNA using the Ambion Illumina cRNA amplification and labeling kit according to manufacturers’ instructions (Ambion, Austin, TX)
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| Contributor(s) |
Xie H, Noh J, Kim J, Jung K, Eun J, Bae H, Lee J, Park W, Nam S |
| Citation(s) |
22496786 |
| Submission date |
Aug 12, 2011 |
| Last update date |
Oct 18, 2022 |
| Contact name |
Jung Woo Eun |
| E-mail(s) |
jetaimebin@catholic.ac.kr
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| Organization name |
Ajou University of Korea
|
| Street address |
Department of Gastroenterology
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| City |
Suwon, Korea |
| State/province |
Korea |
| ZIP/Postal code |
16499 |
| Country |
South Korea |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA145947 |
