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| Status |
Public on Aug 03, 2014 |
| Title |
Gene expression profile of human epidermoid carcinoma KB cells that overexpress thymidine phosphorylase |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Thymidine phosphorylase (TP) catalyzes the reversible phosphorolysis of thymidine, deoxyuridine, and their analogs to the respective bases and 2-deoxy-D-ribose-1-phosphate. TP is identical to platelet-derived endothelial cell growth factor (PD-ECGF). TP was reported to induce many angiogenic factors. However, the molecular mechanism of the TP function is still obscure. To elucidate the molecular basis for the TP function in human epidermoid carcinoma KB cells, we performed a whole genome-wide microarray analysis. Genes upregulated in KB cells overexpressing TP are supposed to represent potential molecular targets of TP in KB cells.
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| Overall design |
KB cells (4 × 10^5) in 5 ml of culture media were cultured for 24 hours, then the growth medium was replaced with serum-free medium. These cells were maintained at 37 ˚C in a humidified atmosphere of 5% CO2 for 12 hours, then these cells were collected for RNA extraction and quantification of the transcripts by microarray analysis.
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| Contributor(s) |
Tabata S, Ikeda R, Akiyama S |
| Citation(s) |
25350954 |
| Submission date |
Aug 18, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Sho Tabata |
| E-mail(s) |
tabatasho@gmail.com
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| Phone |
81-6-6879-8618
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| Organization name |
Osaka University
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| Department |
Institute for Protein Research,ces
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| Street address |
3-2 Yamadaoka
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| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA145665 |
