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| Status |
Public on Aug 25, 2011 |
| Title |
Identify the downstream targets of CHIR99021 and XAV939 in EpiSC using microarray analysis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The requirements for self-renewal differ between EpiSCs and ES cells and the underlying mechanism is largely unknown. Here we show that mouse EpiSCs can be efficiently derived and robustly propagated even from single cells, using two small-molecule inhibitors: CHIR99021 and XAV939.
To better define how CHIR and XAV act together to maintain EpiSC self-renewal, we performed microarray analyses to identify their downstream targets. The data show the genes regulated by addition of CHIR, XAV or both.
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| Overall design |
EpiSCs starved in serum free growth medium (shown in growth protocol) for 12hrs were treated with CHIR (2hrs), XAV(4hrs), or both, after which total RNA was extracted for analysis.
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| Contributor(s) |
Kim H, Tai C, Ying Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 19, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Qilong Ying |
| Organization name |
USC
|
| Lab |
Qilong Ying
|
| Street address |
1425 san pablo st BCC509b
|
| City |
los angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA145481 |
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