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| Status |
Public on Aug 26, 2011 |
| Title |
Role of Cofilin 1 pathway and actin dynamics in nuclear retinoid receptor function |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We have investigated the role of actin dynamics and the effect of actin cytoskeleton modifying agents on retinoid receptor-mediated transactivation. Using Nef, an actin modifying HIV-1 protein, the role of LMK1/CFL1-mediated actin dynamics in receptor function was studied. The effect of Nef expression on transcriptome was investigated following transfection of HEK293 cells with Nef-expressing plasmid. The array data identified Nef-induced inhibition of a number of genes that contain retinoid receptor binding sites in their promoters.
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| Overall design |
The experiment was designed to study the effect of expression of HIV-1 Nef protein on gene expression levels in HEK293 cells. Cells were transfected in three different experiments (each time in duplicate) with Nef expressing plasmid and a plasmid that contained a non-expressing Nef construct (Nef/Stop) as control. The cells were harvested after 36 of transfection and processed for gene array.
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| |
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| Contributor(s) |
Ishaq M, Natarajan V |
| Citation(s) |
21923909 |
| |
| Submission date |
Aug 25, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Jun Yang |
| E-mail(s) |
jyang@mail.nih.gov
|
| Organization name |
SAIC-Frederick, Inc
|
| Street address |
P. O. Box B
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA145391 |
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