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| Status |
Public on Jul 15, 2012 |
| Title |
Expression data from primary human skin fibroblasts treated with vehicle (DMSO) and the antioxidant Trolox (0.5 mM, 96 hrs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Three (3) independent cell cultures for each condition (DMSO and Trolox) were analyzed. The effect of Trolox was checked on the protein level (being an increased protein level of fully assembled mitochondrial complex I) using blue-native PAGE analysis (as described in Koopman et al., Biochim. Biophys. Acta 1777:853-859, 2008, prior to mRNA extraction. We used the control cell line #5120 at similar passage number for all six experiments.
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| Overall design |
We generated the following pairwise comparisons Vehicle > Trolox and Vehicle < Trolox. Changes ≥2 and Benjamini Hochberg corrected p-values <0.05 were considered significant.
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| Contributor(s) |
Distelmaier F, Valsecchi F, Forkink M, van Emst-de Vries S, Swarts HG, Imamura H, Verwiel ET, Rodenburg RJ, Smeitink JA, Willems PH, Koopman WJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 30, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Werner J.H. Koopman |
| Organization name |
Radboud University Nijmegen Medical Centre
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| Department |
286 Biochemistry
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| Street address |
Geert Grooteplein 26-28
|
| City |
Nijmegen |
| State/province |
GLD |
| ZIP/Postal code |
6500 HB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA147755 |
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