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| Status |
Public on Jan 01, 2013 |
| Title |
Genome-wide maps of MEF2C and H3K27ac localization in HUVECs |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
MEF2C is one of the substantially expressed transcriptional factors in endothelial cells, but its genomic localization is unknown. This time, we established a new antibody for MEF2C, and performed ChIP-seq to identify MEF2C binding site in whole genome manner. H3K27Ac binding sites were also detected in the same way.
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| Overall design |
We used chromatin immunoprecipitation with deep sequencing (ChIP-seq) of HUVECs treated with or without pitavastatin for 4hours, we identified MEF2C and H3K27Ac binding regions.HUVECs were used within the first 6 passages. For MEF2C studies, HUVECs were cultivated in medium EGM2MV containing pitavastatin at a concentration of 1 μM, and same concentration of DMSO was used as a control sample. For H3K27ac, HUVECs were starved for 16 hours and harvested without statin treatment.
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| Contributor(s) |
Maejima T, Kohro T, Tsutsumi S, Yamamoto S, Kimura H, Kodama T, Wada Y |
| Citation(s) |
24797675 |
| Submission date |
Oct 05, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Takahide Kohro |
| E-mail(s) |
kohro@lsbm.org
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| Phone |
81-3-5452-5237
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| Organization name |
LSBM, RCAST
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| Street address |
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| City |
4-6-1, Komaba, Meguroku |
| State/province |
Tokyo |
| ZIP/Postal code |
153-8904 |
| Country |
Japan |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (3) |
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| Relations |
| SRA |
SRP008777 |
| BioProject |
PRJNA146949 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32644_RAW.tar |
887.1 Mb |
(http)(custom) |
TAR (of BED, WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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