 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 15, 2012 |
| Title |
Exon arrays from HeLa cells transfected with RBM4-specific siRNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
RBM4 is a RNA-binding protein (RBP) able to modulate splicing by promoting exon inclusion and shares an import pathway with other splicing factors in the nucleus. In earlier studies we found that RBM4 interacts and colocalizes with WT1, which has been implicated in 10–15% of Wilms’ tumours and more recently in leukemya, is considered to be a tumour suppressor. RBM4, a RBP whose splicing effect is inhibited by the +KTS isoform of the tumour-suppressor/activator WT1, exhibits altered expression in different tumours, and may be essential for proliferation. Moreover, RBM4 binds to a number of RNAs of genes involved in acute myeloid leukaemia and cell cycle control. These results suggest that RBM4 may be involved in alternative splicing, tumorigenesis and particularly leukaemogenesis. To determine the endogenous transcripts that are targeted by RBM4 at the genome-wide level, we decided to perform exon microarray studies.
|
| |
|
| Overall design |
RBM4-specific siRNA has been used for knockdown of RBM4 in HeLa cells. RNA was extracted using a Qiagen RNA extraction kit from untransfected, mock and siRNA-transfected HeLa cells and quality of RNA for microarray analysis was checked using a Bioanalyzer 2100 (Agilent Technologies). Experiments were run in triplicates.
|
| |
|
| Contributor(s) |
Markus MA, Marques FZ, Yang YH, Morris BJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 12, 2011 |
| Last update date |
Jul 10, 2014 |
| Contact name |
Prof.Brian J Morris |
| E-mail(s) |
brian.morris@sydney.edu.au
|
| Organization name |
University of Sydney
|
| Department |
Physiology
|
| Lab |
Basic & Clinical Genomics Lab
|
| Street address |
Anderson Stuart Building, room N452, University of Sydney
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
|
| Samples (12)
|
| GSM815585 |
Untransfected cells, biological rep1 |
| GSM815586 |
Untransfected cells, biological rep2 |
| GSM815587 |
Untransfected cells, biological rep3 |
| GSM815588 |
mock, biological rep1 |
| GSM815589 |
mock, biological rep2 |
| GSM815590 |
mock, biological rep3 |
| GSM815591 |
siRNA transfected cells, biological rep1 |
| GSM815592 |
siRNA transfected cells, biological rep2 |
| GSM815593 |
siRNA transfected cells, biological rep3 |
| GSM815594 |
RBM4-specific siRNA transfected cells, biological rep1 |
| GSM815595 |
RBM4-specific siRNA transfected cells, biological rep2 |
| GSM815596 |
RBM4-specific siRNA transfected cells, biological rep3 |
|
| Relations |
| BioProject |
PRJNA146563 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32933_RAW.tar |
265.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...