|
| Status |
Public on Feb 01, 2012 |
| Title |
Scf-GFP+ cells from the bone marrow and whole bone marrow microarray |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The HSC niche factor SCF is required for HSC maintenance. Using an Scf-GFP knockin mouse, we have identified a perivascular cell type in the bone marrow expressing high level of Scf.
To characterize the novel Scf-GFP+ cells from the bone marrow, we performed microarray analysis on these cells.
|
| |
|
| Overall design |
Total RNA were isolated from 3 independent, freshly aliquots of FACS sorted 5,000 SCF-GFP+ cells or whole bone marrow cells isolated from young adult mice. Purified RNA was amplified using the WT-Ovation™ Pico RNA Amplification system (NuGEN Technologies). Sense strand cDNA was generated using WT-Ovation™ Exon Module (NuGEN), then fragmented and labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN). 2.5µg of labeled cDNA were hybridized to Affymetrix Mouse Gene ST 1.0 microarrays.
|
| |
|
| Contributor(s) |
Ding L, Morrison SJ |
| Citation(s) |
22281595, 38012403 |
| Submission date |
Oct 21, 2011 |
| Last update date |
Dec 22, 2023 |
| Contact name |
Lei Ding |
| Organization name |
Columbia University Medical Center
|
| Street address |
630 West 168 ST, P&S, room 7-513
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA149183 |
Less...
More...