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Series GSE33624 Query DataSets for GSE33624
Status Public on Jun 04, 2013
Title Distinct effects of topoisomerase II inhibitors on tumor cell lines (part 1)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells. Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ´open´ or loosely packed chromosomal areas reflecting exon and promoter regions. As a result, the epigenome and the transcriptome are strongly affected.
 
Overall design Tissue culture cells were treated with doxorubicin, aclarubicin or etoposide for 2 hours. Then drugs were removed by extensive washing. cells were further cultured for indicated days before total RNA were extracted and compared to un-treated control.
 
Contributor(s) Pang B, Velds A, Kerkhoven R, Neefjes J
Citation(s) 23715267
Submission date Nov 11, 2011
Last update date Feb 18, 2014
Contact name Baoxu Pang
Organization name Stanford University
Department Genetics
Lab Michael Snyder
Street address 3165 Porter Drive
City Palo Alto
State/province CALIFORNIA
ZIP/Postal code 94304
Country USA
 
Platforms (1)
GPL13376 Illumina HumanWG-6 v2.0 expression beadchip
Samples (14)
GSM831599 MelJuSo cells un-treated
GSM831600 MelJuSo cells 1 day post doxorubicin rep 1
GSM831601 MelJuSo cells 1 day post doxorubicin rep 2
This SubSeries is part of SuperSeries:
GSE33634 Topoisomerase II inhibitors and histone eviction
Relations
BioProject PRJNA154321

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33624_RAW.tar 12.0 Mb (http)(custom) TAR
GSE33624_non-normalized.txt.gz 3.9 Mb (ftp)(http) TXT
GSE33624_normalized.txt.gz 5.5 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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