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| Status |
Public on Jun 04, 2013 |
| Title |
Epigenetic changes due to histone eviction induced by anthracycline doxorubicin and aclarubicin |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells. Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ´open´ or loosely packed chromosomal areas reflecting exon and promoter regions.
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| Overall design |
Comparison of histone H3K4me3 of cells post topoisomerase II inhibitors treatment to un-treated ones by ChIP-seq.
Comparison of phosphorylated histone H2AX of cells post topoisomerase II inhibitors doxorubicin and etoposide treatment to un-treated ones by ChIP-seq.
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| Contributor(s) |
Zwart W, Pang B, Velds A, Neefjes J |
| Citation(s) |
23715267 |
| Submission date |
Nov 11, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Baoxu Pang |
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Lab |
Michael Snyder
|
| Street address |
3165 Porter Drive
|
| City |
Palo Alto |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (3) |
| GSM831874 |
MelJuSo un-treated H3K4me3 ChIP-seq |
| GSM831875 |
MelJuSo doxorubicin H3K4me3 ChIP-seq |
| GSM831876 |
MelJuSo etoposide H3K4me3 ChIP-seq |
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| This SubSeries is part of SuperSeries: |
| GSE33634 |
Topoisomerase II inhibitors and histone eviction |
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| Relations |
| SRA |
SRP009323 |
| BioProject |
PRJNA154319 |
