 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 12, 2012 |
| Title |
TCERG1 regulates alternative splicing of Bcl-x gene by modulating the rate of RNAPII transcription |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Complex functional coupling exists between transcriptional elongation and pre-mRNA alternative splicing. Pausing sites and changes in the rate of transcription by RNAPII may therefore have a fundamental impact in the regulation of alternative splicing. Here, we show that the elongation and splicing-related factor TCERG1 regulates alternative splicing of the apoptosis gene Bcl-x in a promoter-dependent manner. TCERG1 promotes the splicing of the short isoform of Bcl-x (Bcl-xs) through the SB1 regulatory element located in the first half of exon 2. Consistent with these results, we show evidence for in vitro and in vivo interaction of TCERG1 with the Bcl-x pre-mRNA. Transcription profile analysis reveals that the RNA sequences required for the effect of TCERG1 on Bcl-x alternative splicing coincide with a putative polymerase pause site. Furthermore, TCERG1 modifies the impact of a slow polymerase on Bcl-x alternative splicing. In support of a role for an elongation mechanism in the transcriptional control of Bcl-x alternative splicing, we found that TCERG1 modifies the amount of pre-mRNAs generated at distal regions of the endogenous Bcl-x. Most importantly, TCERG1 affects the rate of RNAPII transcription of endogenous human Bcl-x. We propose that TCERG1 modulates the elongation rate of RNAPII to relieve pausing, thereby activating the pro-apoptotic Bcl-xS 5’ splice site.
|
| |
|
| Overall design |
ChIP-Seq
|
| |
|
| Contributor(s) |
Montes M, Cloutier A, Sánchez-Hernández N, Michelle L, Lemieux B, Blanchette M, Hernández-Munain C, Chabot B, Suñé C |
| Citation(s) |
22158966 |
| Submission date |
Nov 15, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Marco Blanchette |
| E-mail(s) |
marcoblanchette@icloud.com
|
| Phone |
415-272-4837
|
| Organization name |
Stowers Institute for Medical Research
|
| Lab |
Marco Blanchette
|
| Street address |
1000 E. 50th St.
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (1) |
|
| Relations |
| SRA |
SRP009342 |
| BioProject |
PRJNA148459 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33716_RAW.tar |
413.8 Mb |
(http)(custom) |
TAR (of BAM) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
