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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 26, 2011 |
| Title |
Gene expression regulated by AGN193109 and 4-diehtylaminobenzaldehyde (DEAB) in mIMCD-3 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
All-trans retinoic acid (tRA) is the bioactive derivative of vitamin A that regulates gene expression by activating RA receptors (RAR). By using a reporter mouse model, we recently showed that endogenous tRA/RAR signaling was present in kidney collecting duct cells, and in mouse inner medullary collecting duct cell line (mIMCD-3).
In order to unveil target genes of endogenous tRA/RAR signaling in kidney collecting duct cells, whole genome microarray analysis was performed on mIMCD-3 cells treated with AGN193109, a pan-RAR antagonist, and 4-diethylaminobenzaldehyde (DEAB), an inhibitor of tRA synthesizing enzyme. Specificity of gene expression regulation was confirmed by determining the reversibility of the regulation by simultaneous addition of exogenous tRA.
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| Overall design |
In the first set of experiment, mIMCD-3 cells were treated with either vehicle (0.1% ethanol and 0.1% DMSO) or 1 μM AGN193109 or 1 μM AGN193109+0.2 μM tRA. Three independent biological experiments were performed. In the second set of experiment, mIMCD-3 cells were treated with either vehicle (0.1% ethanol and 0.1% DMSO) or 25 μM DEAB or 25 μM DEAB+0.01 μM tRA. Three independent biological experiments were performed. Treatment period for both sets of experiments were 24 hours.
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| Contributor(s) |
Wong Y, Xu Q |
| Citation(s) |
23049847 |
| Submission date |
Nov 25, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Qihe Xu |
| E-mail(s) |
qihe.xu@kcl.ac.uk
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| Organization name |
King's College London
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| Street address |
123, Coldharbour Lane
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| City |
London |
| ZIP/Postal code |
SE5 9NU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA150549 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33955_RAW.tar |
85.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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