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| Status |
Public on Dec 01, 2015 |
| Title |
Deep sequencing analysis of small non-coding RNAs reveals the diversity of microRNAs and piRNAs in the human epididymis. |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The epididymis in male reproductive system plays a crucial role in regulating the development of sperm motility and fertilizing capacity. Small non-coding RNAs (sncRNAs), especially microRNAs (miRNAs), can participate in the regulation of various physiological pathways. However, their abundance and whether they are involved in the regulation of gene expression in the human epididymis are unknown. By adopting the Solexa deep sequencing approach, we systematically investigated the sncRNAs in the adult human epididymis. A total of 4,903 unique reads representing 527 known miRNA were discovered. Eighteen novel miRNA genes encoding 23 mature miRNAs were also identified, suggesting a complicated miRNA regulatory network. The expression of some of the novel miRNA was confirmed by qRT-PCR. The presence of Piwi-interacting RNAs (piRNAs) in the library also adds to the diversity of sncRNA population in the human epididymis. This research lays the foundation for the functional study of sncRNAs in huamn reproductive system.
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| Overall design |
The epididymis from a 26-year-old male who is healthy and fertile was deep-sequenced.
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| Contributor(s) |
Li Y, Wang H, Wan F, Liu F, Liu J, Zhang N, Jin S, Li J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 28, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Yan Li |
| E-mail(s) |
liyan0535@yahoo.com.cn
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| Organization name |
Shandong Research Centre for Stem Cell Engineering
|
| Street address |
20th, Yu Dong Road, Zhifu Area
|
| City |
Yantai |
| State/province |
Shandong |
| ZIP/Postal code |
264000 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| SRA |
SRP009455 |
| BioProject |
PRJNA150539 |
