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| Status |
Public on Nov 30, 2011 |
| Title |
Expression data from third instar Drosophila larvae offspring from maternal control or high-sucrose fed diets |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Maternal obesity and diabetes is associated with increased risk of obesity and diabetes in offspring. We generated a model of maternal caloric excess in Drosophila and noted altered body composition in offspring from females fed a high-sucrose diet. To gain insight into the mechanisms underlying this response, we profiled gene expression in mid-third instar larvae (mid-L3) offspring from either control or high-sucrose fed females. All offspring were raised on control food.
We used microarrays to detail the response of Drosophila larvae to maternal high calorie diet
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| Overall design |
Virgin female w1118 flies were fed control (0.15M) or high (1M) sucrose food for 7 days, mated with male w1118 flies such that all embryos were laid on control food. Mid-L3 larvae were selected for RNA extraction and hybridization on Affymetrix microarrays. Mid-L3 were selected as L2, aged overnight until early L3, then transferred to fresh control food for 12 more hours before selection.
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| Contributor(s) |
Duncan JG, Musselman LP |
| Citation missing |
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| Submission date |
Nov 29, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jennifer G Duncan |
| E-mail(s) |
duncan_j@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pediatrics
|
| Lab |
Duncan
|
| Street address |
660 S. Euclid, Box 8208
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63124 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| GSM840105 |
wandering L3 control reared biological rep 1 |
| GSM840106 |
wandering L3 control reared biological rep 2 |
| GSM840107 |
wandering L3 control reared biological rep 3 |
| GSM840108 |
wandering L3 high sugar reared biological rep 1 |
| GSM840109 |
wandering L3 high sugar reared biological rep 2 |
| GSM840110 |
wandering L3 high sugar reared biological rep 3 |
|
| Relations |
| BioProject |
PRJNA150609 |
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