|
| Status |
Public on May 31, 2012 |
| Title |
Transcriptomic analysis of Etoposide-treated 293 cells expressing p65 wt or p65 S547A |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We reported a direct phosphorylation of the Ser547 of the RelA/p65 subunit of NF-kB by ATM kinase. A comparative transcriptomic analyse with HEK293 cells expressing exclusively HA-p65wt or a non-phosphorylable mutant HA-p65S547A was conducted to identify the genes regulated by this phosphorylation after an Etoposide treatment.
|
| |
|
| Overall design |
HEK293 cells expressing exclusively HA-p65wt or HA-p65S547A were treated during four or eight hours with Etoposide or left untreated. This was performed three times to obtain independent triplicates for the microarray expermiment.
|
| |
|
| Contributor(s) |
Sabatel H, Divalentin E, Gloire G, Dequiedt F, Piette J, Habraken Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 29, 2011 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Helene Sabatel |
| E-mail(s) |
helene.sabatel@student.ulg.ac.be
|
| Organization name |
Universite de Liege
|
| Street address |
avenue de l'hopital
|
| City |
Liege |
| ZIP/Postal code |
4000 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA150633 |
Less...
More...