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| Status |
Public on Jul 01, 2013 |
| Title |
PRC2 is required for acute myeloid leukemias initiated by MLL-AF9 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcriptional activating and repressive functions performed by Trithorax and Polycomb group complexes, respectively, are critical for to maintain cellular fates in ontogeny and in cancer. Here we report that leukemias initiated by a Trithorax-related oncogene, MLL-AF9, depend upon the Polycomb Repressive Complex 2 (PRC2) to sustain a transformed cellular state. RNAi mediated suppression of PRC2 subunits is sufficient to inhibit proliferation of MLL-AF9 leukemias, with little impact on growth of non-transformed cells. This requirement is partly due to PRC2-mediated transcriptional repression of several anti-self-renewal regulators, including Cdkn2a. These results suggest that, unlike the classical antagonism generally observed between Polycomb and Trithorax group proteins during development, the activities of these two pathways can cooperate to promote myeloid neoplasia.
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| Overall design |
In order to understand downstream targets of PRC2 complex in MLL-AF9 leukemia, we performed array in murine MLL-AF9/NrasG12D cell line under the condition that two subunits of PRC2(Eed and Suz12) were suppressed by using shRNAs.
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| Contributor(s) |
Shi J, Wang E, Zuber J, Johns C, Lowe SW, Vakoc CR |
| Citation(s) |
22469984 |
| Submission date |
Dec 06, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Junwei Shi |
| Organization name |
cold spring harbor lab
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM843549 |
MLL-AF9/Nras LMN Eedsh day5 sample1 |
| GSM843550 |
MLL-AF9/Nras LMN Eedsh day5 sample2 |
| GSM843551 |
MLL-AF9/Nras LMN Eedsh day5 sample3 |
| GSM843552 |
MLL-AF9/Nras LMN Suz12sh day5 sample1 |
| GSM843553 |
MLL-AF9/Nras LMN Suz12sh day5 sample2 |
| GSM843554 |
MLL-AF9/Nras LMN Suz12sh day5 sample3 |
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| Relations |
| BioProject |
PRJNA149697 |
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