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| Status |
Public on Nov 02, 2005 |
| Title |
Silencing of microRNAs in vivo with "antagomirs" |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Using a novel class of chemically-engineered oligonucleotides, termed "antagomirs", we studied the biological significance of silencing miR-122 in the liver of mice at the mRNA level
Keywords: treatment response
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| Overall design |
Mice were injected on three subsequent days with antagomir-122 or scrambled control (mm-antagomir-122), respectively; 80mg/kg bodyweight/day. 24 hrs after the last treatment, mice were sacrificed and total RNA isolated from the livers. RNA from three mice was pooled and used for generation of biotin-labelled cRNA.
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| Contributor(s) |
Krutzfeldt J, Rajewsky N, Braich R, Rajeev KG, Tuschl T, Manoharan M, Stoffel M |
| Citation(s) |
16258535 |
| Submission date |
Oct 07, 2005 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Markus Stoffel |
| E-mail(s) |
stoffel@rockefeller.edu
|
| Phone |
212-327-8797
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| Fax |
212-327-7997
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| Organization name |
Rockefeller University
|
| Department |
Laboratory of Metabolic Diseases
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| Street address |
1230 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA93459 |
