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| Status |
Public on Nov 01, 2012 |
| Title |
miRNA transcript levels in ccRCC-derived cell lines and proximal tubular epithelial cell samples |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Despite numerous studies reporting deregulated microRNA (miRNA) and gene expression patterns in clear cell renal cell carcinoma (ccRCC), no direct comparisons have been made to its presumed normal counterpart; the renal proximal epithelial tubular cells (PTEC). The aim of this study was to determine the miRNA expression profiles of ten clear cell renal cell carcinoma-derived cell lines and short-term cultures of PTEC, and to correlate these with their gene expression, and copy-number profiles. Using microarray-based methods, a significantly altered expression level in ccRCC cell lines was observed for 23 miRNAs and 1630 genes. The set of miRNAs with significantly decreased expression levels include all members of the miR-200 family known to be involved in the epithelial to mesenchymal transition (EMT) process. Expression levels of 13 of the 47 validated target genes for the downregulated miRNAs were increased more than two-fold. Our data reinforce the importance of the EMT process in the development of ccRCC. For mRNA expression data of these cell lines see GEO Series accession number GSE20491.
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| Overall design |
MicroRNA profiling was performed on two proximal tubular epithelial cell samples (both cell samples were hybridized twice (biological duplicates)) and ten clear cell renal cell carcinoma- derived cell lines (one of which; RCC-JF in duplicate)
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| Contributor(s) |
Duns G, Kok K, Van Duivenbode I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 10, 2012 |
| Last update date |
May 07, 2014 |
| Contact name |
Klaas Kok |
| E-mail(s) |
k.kok@umcg.nl
|
| Phone |
+31(0)632724387
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| Organization name |
UMCG
|
| Department |
Genetics
|
| Street address |
PO box 30.001
|
| City |
Groningen |
| ZIP/Postal code |
9700 RB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL8227 |
Agilent-019118 Human miRNA Microarray 2.0 G4470B (miRNA ID version) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE34985 |
Analysis of ccRCC derived cell lines |
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| Relations |
| Affiliated with |
GSE20491 |
| BioProject |
PRJNA156303 |
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