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| Status |
Public on Feb 15, 2012 |
| Title |
Analysis of global gene expression profiles of hPAF1 deficiency on unstimulated A549 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Viral infection is commonly associated with virus-driven hijacking of host proteins. We describe a novel mechanism by which influenza virus impacts host cells through the interaction of influenza NS1 protein with the infected cell epigenome. We show that the NS1 protein of influenza A H3N2 targets the transcription elongation PAF1 complex (hPAF1C). We demonstrate that binding of NS1 to hPAF1C results in suppression of hPAF1C-mediated transcriptional elongation.
More importantly,in the following data sets, we show that hPAF1 plays a crucial role in the antiviral response. Loss of hPAF1C reduces antiviral gene expression and reduces inducible transcription of target genes after stimulation with viral RNA analogue poly(I:C), vesicular stomatitis virus (VSV), exogenous recombinant IFN(beta) and influenza virus (H1N1). This study underscores the importance of hPAF1C in controlling inducible antiviral gene expression.
A549 cells were untreated (no siRNA) or treated with control siRNA-treated and hPAF1 siRNA. Total RNA was isolated with the Qiagen RNeasy mini kit. 200ng of total RNA per sample was used to prepare biotin-labeled RNA using MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems) and hybridized to HumanHT-12 v4 Expression BeadChips (Illumina). Data analysis was performed using the GeneSpring GX11.0 software (Agilent Technologies). These samples were then used for comparisons with stimulated cells (See series 1)
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| Overall design |
3 biological replicates per condition
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| Contributor(s) |
Marazzi I, Ho J, Kim J, Manicassamy B, Dewell S, Albrecht RA, Seibert C, Schaefer U, Jeffrey KL, Prinjha RK, Lee K, García-Sastre A, Roeder R, Tarakhovsky A |
| Citation(s) |
22419161 |
| Submission date |
Jan 23, 2012 |
| Last update date |
Jul 29, 2020 |
| Contact name |
ivan marazzi |
| E-mail(s) |
imarazzi@rockefeller.edu
|
| Phone |
2123278265
|
| Fax |
2123278258
|
| Organization name |
rockefeller univeristy
|
| Lab |
epigenetic and immune signaling
|
| Street address |
1230 York Avenue
|
| City |
new york |
| State/province |
ny |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE35268 |
Analysis of global gene expression profiles of hPAF1 deficient A549 cells |
|
| Relations |
| BioProject |
PRJNA156043 |
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