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Status |
Public on Jul 24, 2012 |
Title |
Genome-wide analysis of gene expression after tyrosine or methionine/cysteine deprivation in HeLa cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Limitation of essential amino acids, such as tyrosine or methionine/cysteine, causes upregulation of exogenous integrated transgene expression in mammalian cells. This phenomenon is mediated by histone acetylation and chromatin remodelling, since histone deacetylase (HDAC) inhibitors reproduce starvation-induced transgene upregulation and chromatin immunoprecipitation analysis of amino acid-deprived cells reveals significant changes in total core histones detectable at the CMV promoter. Expression profiling of HeLa cells starved for 5 days in medium without tyrosine or methionine/cysteine provides important information on the cellular response to amino acid deprivation and suggests the involvement of HDAC4 (class II HDAC) in transgene derepression during amino acid starvation.
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Overall design |
Total RNA obtained from HeLa and HeLaOA1myc cells (HeLa cells with a human ocular albinism type 1 (OA1)+myc tag transgene) subjected to 5 days of tyrosine or methionine/cysteine starvation compared to control cells. Twelve samples are analyzed: HeLa and HeLaOA1myc grown for 5 days in RPMI deprived of tyrosine (Y) vs. complete RPMI; technical duplicates of HeLa and HeLaOA1myc grown for 5 days in DMEM deprived of methionine/cysteine (MC) vs. complete DMEM.
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Contributor(s) |
Palmisano I, Brambilla P, Boneschi FM, Schiaffino MV |
Citation(s) |
22826225 |
Submission date |
Jan 24, 2012 |
Last update date |
Feb 18, 2019 |
Contact name |
Ilaria Palmisano |
E-mail(s) |
palmisano.ilaria@hsr.it
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Organization name |
San Raffaele Scientific Institute
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Department |
Center for Translational Genomics and BioInformatics
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Street address |
Via Olgettina, 58
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City |
Milan |
ZIP/Postal code |
20132 |
Country |
Italy |
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Platforms (1) |
GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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Samples (12)
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Relations |
BioProject |
PRJNA151551 |