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| Status |
Public on Jan 31, 2012 |
| Title |
Genome-wide analysis of gene expression changes in miR-214 KO mouse hearts and skeletal muscle |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Early reperfusion of ischemic cardiac tissue remains the most effective intervention for improving clinical outcome following myocardial infarction. However, abrupt increases in intracellular Ca2+ during myocardial reperfusion cause cardiomyocyte death and consequent loss of cardiac function, referred to as ischemia/reperfusion (IR) injury. Cardiac IR is accompanied by dynamic changes in expression of microRNAs (miRNAs), which inhibit specific mRNA targets. miR-214 is up-regulated during ischemic injury and heart failure in mice and humans, but its potential role in these processes is unknown. We show that genetic deletion of miR-214 in mice causes loss of cardiac contractility, increased apoptosis, and excessive fibrosis in response to IR injury.
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| Overall design |
The microarray contains 6 samples, each containing cDNA pooled from 3 mice per group. There are no replicates. The array was designed to make 3 different pairwise comparisons between the following: P14 WT and miR-214 KO hearts; adult WT and miR-214 KO skeletal muscle; adult WT and miR-214 KO hearts
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| |
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| Contributor(s) |
Aurora AB, Olson EN |
| Citation(s) |
22426211 |
| Submission date |
Jan 30, 2012 |
| Last update date |
Sep 23, 2019 |
| Contact name |
Arin B Aurora |
| E-mail(s) |
arinaurora@gmail.com
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Molecular Biology
|
| Lab |
Eric N. Olson
|
| Street address |
6000 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75235 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA152583 |
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