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| Status |
Public on Jan 01, 2018 |
| Title |
Small RNA profiles through the cell cycle in HeLa [small RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Dicer plays a key role in RNA silencing. By processing pre-miRNAs and dsRNAs, Dicer generates miRNAs and siRNAs that act as post-transcriptional regulators of gene expression. Dicer is also implicated in heterochromatin formation and toxic RNA degradation. Here we report that Dicer is controlled in cell cycle. The Dicer protein level drastically rises at late G1 phase and falls at early S phase. Interestingly, the protein stability of Dicer is decreased specifically at early S phase. MG132, an inhibitor of proteasome, increases Dicer protein level, suggesting that the stability of Dicer is controlled via ubiquitination-dependent proteasome pathway.
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| Overall design |
All samples were first synchronized to early S phase by thymidine double block, then RNA were extracted from the cells after 8, 10, 14, 16, 18 or 20 hours from releasing the cell cycle progression.
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| Contributor(s) |
Lee JH, Chang H, Heo I, Park J, Kim VN |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 03, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Hyeshik Chang |
| E-mail(s) |
hyeshik@snu.ac.kr
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| Organization name |
Seoul National University
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| Department |
School of Biological Sciences
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| Lab |
Hyeshik Chang Lab
|
| Street address |
Building 203 Room 525, School of Biological Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu
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| City |
Seoul |
| State/province |
South Korea |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE35533 |
Analysis of small RNA through the cell cycle in HeLa |
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| Relations |
| SRA |
SRP010745 |
| BioProject |
PRJNA156091 |
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