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| Status |
Public on Mar 19, 2015 |
| Title |
Gene Expression profiles of Bapx1 sorted cells from vertebral column of wildtype (WT) and Bapx1 null (HOMO) E12.5 mouse embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
This study aims to look at gene expresion profiles between wildtype and Bapx1 knockout cells of the vertebral column in a E12.5 mouse embryo. Instead of looking at the whole vertebral column ,only cells expressing Bapx1 were sorted by Fluroscent Activated Cell Sorting (FACS) and subjected to expression profiling by microarray.
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| Overall design |
EGFP was knocked into the Bapx1 locus by homologous recombination in mESC followed by generation of stable mice line. Wildtype and homozygous embryos expressing EGFP was was used to dissected out the vertebral column and Fluroscent activated cell sorting was used to sort out cell expressing Bapx1 as marked by EGFP. Total RNA was extracted and hybidized to an Illumina WG6 v2 mouse array.
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| Contributor(s) |
Lufkin T |
| Citation(s) |
25480362 |
| Submission date |
Feb 09, 2012 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Thomas Lufkin |
| E-mail(s) |
tlufkin@clarkson.edu
|
| Organization name |
Clarkson University
|
| Department |
Biology
|
| Lab |
Stem Cells & Regenerative Medicine
|
| Street address |
8 Clarkson Avenue
|
| City |
Potsdam |
| State/province |
NY |
| ZIP/Postal code |
13699 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (8)
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| GSM873005 |
WTBapx1 Vertebral Column Cells-Rep1 |
| GSM873006 |
WTBapx1 Vertebral Column Cells-Rep2 |
| GSM873007 |
WTBapx1 Vertebral Column Cells-Rep3 |
| GSM873008 |
WTBapx1 Vertebral Column Cells-Rep4 |
| GSM873009 |
HomoBapx1 Vertebral Column Cells-Rep1 |
| GSM873010 |
HomoBapx1 Vertebral Column Cells-Rep2 |
| GSM873011 |
HomoBapx1 Vertebral Column Cells-Rep3 |
| GSM873012 |
HomoBapx1 Vertebral Column Cells-Rep4 |
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| This SubSeries is part of SuperSeries: |
| GSE35877 |
Genome wide binding and expression profiling defines a Bapx1-Sox9 axis in vertebral column, Gut, Spleen and limbs in developing mouse embryo. |
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| Relations |
| BioProject |
PRJNA155859 |
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