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| Status |
Public on Feb 12, 2018 |
| Title |
Tumor suppressor REST/NRSF controls genome stability by preventing R-loop formation |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Analysis of HeLa cell with overexpression of an EGFP-tagged dominant-negative truncation (73- 563 a.a.) of human REST/NRSF. RNA polymerase II and DNA damage repair factor γ-H2AX binding data provide insight into the molecular bases of how REST/NRSF perturbation impairs genome stability.
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| Overall design |
Identification of RNA polymerase II and γ-H2AX binding sites in normal and REST-disrupted HeLa cells. An antibody targeting the YSPTSPS repeats in the C-terminal domain (CTD) of the largest subunit of RNAP II was utilized to immunoprecipitate RNAP II, regardless of its phosphorylation status.
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| |
|
| Contributor(s) |
Wei Y, Yang F, Tu J, Zhao P, Zhai Y, Tao Y, Li X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 21, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
XiaLu Li |
| E-mail(s) |
6239@cnu.edu.cn
|
| Organization name |
Capital Normal University
|
| Street address |
105 Xisanhuan Beilu
|
| City |
beijing |
| ZIP/Postal code |
100037 |
| Country |
China |
| |
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| Platforms (2) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (4)
|
| GSM878156 |
HeLa (p77-infected) RNA polymerase II ChIP-seq |
| GSM878157 |
HeLa (pLL3.7-infected) RNA polymerase II ChIP-seq |
| GSM878158 |
HeLa (p77-infected) γ-H2AX ChIP-seq |
| GSM878159 |
HeLa (pLL3.7-infected) γ-H2AX ChIP-seq |
|
| Relations |
| SRA |
SRP011003 |
| BioProject |
PRJNA152061 |
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