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| Status |
Public on Feb 22, 2012 |
| Title |
A novel role of stem cell factor SALL4 as an oncofetal protein in hepatocellular carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Treatment of HCC remains abysmal; discovery of novel pathways implicated in hepatocarcinogenesis is needed for more effective therapeutics. SALL4 is a stem cell factor essential for the maintenance of embryonic stem cell self-renewal properties and expressed in fetal liver, but silenced in normal adult liver. We observed that expression of SALL4 in murine liver in a transgenic model led to development of HCC. In humans, SALL4 is re-expressed as an oncofetal protein in a subgroup of HCC patients with unfavorable prognoses. Loss-of-function studies demonstrated that SALL4 is essential for human HCC cell survival and tumorigenicity.
We demonstrated that a peptide can block the oncogenic function of SALL4 in HCC by modulating the PTEN/AKT pathway. Our findings reveal a novel role of SALL4 in HCC with important implications for understanding disease mechanisms and development of innovative therapeutics.
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| Overall design |
Cultured cells from HCC cell line SNU-398 with scrambled or SALL4 shRNA knockdown were used for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Yong KJ, Gao C, Yan B, Yang H, Lim J, Dimitrov T, Kawasaki A, Ong CW, Lee S, Ravikumar S, Srivastava S, Tian X, Luk JM, Dan YY, Salto-Tellez M, Chai L, Tenen DG |
| Citation missing |
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| Submission date |
Feb 21, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Henry Yang |
| E-mail(s) |
csiyangh@nus.edu.sg
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| Organization name |
Cancer Science Institute of Singapore
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| Street address |
28 Medical Drive
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| City |
Singapore |
| ZIP/Postal code |
117456 |
| Country |
Singapore |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA152063 |
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