|
| Status |
Public on Jun 10, 2012 |
| Title |
Genome-wide mRNA expression profiles induced by infection with the pandemic and seasonal influenza A viruses in macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Infection with pandemic A/H1N1 influenza virus induces high levels of circulating and lung pro-inflammatory cytokines and chemokines in experimental models and humans. These inflammatory mediators contribute to poor clinical outcomes of this infection. To compare the regulation of inflammatory responses in seasonal versus pandemic influenza A virus infections, we analyzed the expression of mRNA by the human gene st 1.0 array (affymetrix). We identifed a number of differentially expressed genes by infection of the A/H1N1 compared to the seasonal virus. Gene ontology analysis of these genes found that most of them are involved in several aspects of immunity. These studies suggest that factors inherent in the pandemic A/H1N1 viral strain may augment the production of inflammatory mediators by inhibiting SOCS- 1 expression and that infection with this viral strain also modifies the expression of several immunity related genes and pathways .
|
| |
|
| Overall design |
The experimental design consist in 1 pool and its replicate containing 5 independent experiments from treated macrophages with Influenza A/H1N1 virus, and 1 more pool and its replicate containing 5 independent experiments from treated macrophages with seasonal influenza A virus. By this way, the total number of the samples analyzed was 10 in 4 microarrays.
|
| |
|
| Contributor(s) |
Ramírez G, Cruz-Lagunas A, Jiménez-Alvarez L, Espinosa E, Ortiz-Quintero B, Santos-Mendoza T, Torres M, Herrera T, Canché-Pool E, Cabello C, Orozco L, Aguilar I, Hidalgo-Miranda A, Romero-Cordoba S, Tager AM, Suratt BT, Pardo A, Selman M, Zuñiga J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 22, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Sandra Lorena Romero-Cordoba |
| E-mail(s) |
sromero_cordoba@hotmail.com
|
| Organization name |
National Instute of Genomic Medicine
|
| Department |
Cancer Genomics
|
| Street address |
Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan. México, D.F.
|
| City |
Mexico |
| State/province |
Mexico |
| ZIP/Postal code |
14610 |
| Country |
Mexico |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA151715 |
Less...
More...