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| Status |
Public on May 01, 2012 |
| Title |
shRNA mediated knockdown of TRA2B and SRSF3 in HEK293 cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have independently knocked down TRA2B and SRSF3 in HEK293 cells to identify the sum of alternative splicing changes that results from depletion of each factor. Each was compared to a control transfection of the hairpin vector alone.
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| Overall design |
In this set, 9 total samples were analyzed. There were three biological replicates for each of three conditions: knockdown control, TRA2B knockdown and SRSF3 knockdown. Arrays were analyzed for gene expression using GeneBase and for alternative splicing using MADS+ and OmniViewer software.
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| Contributor(s) |
Anderson ES, Lin C, Xiao X, Stoilov P, Burge CB, Black DL |
| Citation(s) |
22456266 |
| Submission date |
Feb 24, 2012 |
| Last update date |
Jun 21, 2012 |
| Contact name |
Erik S Anderson |
| E-mail(s) |
esa2@ucla.edu
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| Organization name |
UCLA
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| Department |
MBIDP
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| Lab |
Douglas Black
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| Street address |
6-567 MRL, 675 Charles E. Young Drive South
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL15106 |
[hjay] Affymetrix GeneChip Human HJAY Array |
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| Samples (9)
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| GSM880689 |
control knockdown, biological replicate 1 |
| GSM880690 |
control knockdown, biological replicate 2 |
| GSM880691 |
control knockdown, biological replicate 3 |
| GSM880692 |
Tra2-beta knockdown, biological replicate 1 |
| GSM880693 |
Tra2-beta knockdown, biological replicate 2 |
| GSM880694 |
Tra2-beta knockdown, biological replicate 3 |
| GSM880695 |
SRp20 knockdown, biological replicate 1 |
| GSM880696 |
SRp20 knockdown, biological replicate 2 |
| GSM880697 |
SRp20 knockdown, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA151827 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36069_RAW.tar |
291.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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