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| Status |
Public on Dec 31, 2012 |
| Title |
Genome-wide analysis of gene expression in human embryonic stem cells (hESCs) and their differentiated cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In order to investigate the gene expression changes in human embryonic stem cells (hESCs) during differentiation, we performed a microarray analysis from RNAs isolated from undifferentiated hESCs and their differentiated cells incubated for 1 week or 2 weeks in ESC medium.
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| Overall design |
Human SNUhES3 ESCs (Seoul National University Hospital, Seoul, Korea) were cultured on mitotically-arrested STO feeder cells (ATCC, Manassas, USA) in DMEM/F12 supplemented with 20% knockout serum replacement (KSR). The embryoid bodies were incubated for 1 or 2 weeks in the above ESC medium without bFGF. Cells were then lysed and RNA was isolated.
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| Contributor(s) |
Hwang D, Kim KP, Kim DW, Kang JW, Jeong H, Ha HY, Yun S, Kim JK, Park JH, Cho KC, Kang GY, Jung JW, Park JS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 04, 2012 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Hyobin Jeong |
| E-mail(s) |
h.jeong@imb-mainz.de
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| Organization name |
IMB
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| Street address |
Richard shirrmann Str 12.
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| City |
Mainz |
| State/province |
Germany |
| ZIP/Postal code |
55112 |
| Country |
Germany |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA153073 |
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