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| Status |
Public on Jul 06, 2012 |
| Title |
Gene expression analysis of pluripotent and differentiated genes during EB formation treated with streptozotocin, o-glacnacylation inhibitor |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
O-linked-N-acetylglucosamine (O-GlcNAc) has emerged as a critical regulator of diverse cellular processes, but its role in embryonic stem cells (ESCs) and pluripotency has not been investigated. Here we show that O-GlcNAcylation directly regulates core components of the pluripotency network. Blocking O-GlcNAcylation disrupts ESC self-renewal and reprogramming of somatic cells to induced pluripotent stem cells. The core reprogramming factors Oct4 and Sox2 are O-GlcNAcylated in ESCs, but the O-GlcNAc modification is rapidly removed upon differentiation. O-GlcNAc modification of Threonine 228 in Oct4 regulates Oct4 transcriptional activity and is important for inducing many pluripotency related genes, including Klf2, Klf5, Nr5a2, Tbx3 and Tcl1. A T228A point mutation that eliminates this O-GlcNAc modification reduces the capacity of Oct4 to maintain ESC self-renewal and reprogram somatic cells. Overall, our study makes a direct connection between O-GlcNAcylation of key regulatory transcription factors and the activity of the pluripotency network.
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| Overall design |
2 of E14 stem cell, 2 of embryonic body at day 2, 2 of embryonic body at day 2 treated with streptozotocin, 1 of ZHBTc4 stem cell treated with doxicyclin, and 1 of ZHBTc4 stem cell treated with doxicyclin and streptozotocin were analysed
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| Contributor(s) |
Youn H, Jang H, Kim TW |
| Citation(s) |
22608532 |
| Submission date |
Mar 07, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Taewan Kim |
| E-mail(s) |
taewan79@snu.ac.kr
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| Phone |
082-2-3668-7629
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| Organization name |
Seoul National University of medicine
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| Department |
biomedical science
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| Lab |
metabolic checkpoint lab
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| Street address |
biomedical science building 202ho
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| City |
Youngun dong, jongro gu,Seoul |
| ZIP/Postal code |
110-799 |
| Country |
South Korea |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (8)
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| GSM888897 |
E14 stem cell, undiffrentiated state, rep 1 |
| GSM888898 |
E14 stem cell, undiffrentiated state, rep 2 |
| GSM888899 |
EB2, embryonic body formation for 2 days, rep 1 |
| GSM888900 |
EB2, embryonic body formation for 2 days, rep 2 |
| GSM888901 |
EB2, embryonic body formation for 2 days with streptozotocin, rep 1 |
| GSM888902 |
EB2, embryonic body formation for 2 days with streptozotocin, rep 2 |
| GSM888903 |
ZHBTc4 stem cell with dox treatment, rep1 |
| GSM888904 |
ZHBTc4 stem cell with dox and streptozotocin treatment, rep1 |
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| Relations |
| BioProject |
PRJNA153369 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36322_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE36322_non-normalized_data.txt.gz |
12.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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