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| Status |
Public on Mar 22, 2013 |
| Title |
Whole genome expression after modulation of miR-30a expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
A subset of breast cancer cells displays increased ability to self-renew and reproduce breast cancer heterogeneity (so called, breast tumour-initiating cells or BT-ICs). As microRNAs (miRNAs) control developmental programs in stem cells, BT-ICs may also rely on specific miRNA profiles for their sustained activity. We analyzed miRNA expression in a model of putative BT-ICs and found that miR-30 family regulates growth under “stemness” conditions. A target screening revealed that miR-30 family modulates the expression of apoptosis and proliferation-related genes. The importance of miR-30 in tumour progression and breast cancer stemness was demonstrated in vivo using a mouse mammary cancer model. This is the first analysis of target prediction in a whole family of microRNAs potentially involved in survival of putative BT-ICs.
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| Overall design |
Total RNA from cells transfected with Pre-miR-30 and KD-miR-30 family and control KD-miR-159 was extracted and reverse-transcribed and hybrized on HT12 Human bead chips
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| Contributor(s) |
Hernandez-Vargas H |
| Citation(s) |
23445407 |
| Submission date |
Mar 16, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Hector Hernandez-Vargas |
| E-mail(s) |
hector.hernandez@genomicsconsulting.eu
|
| Organization name |
Centre Leon Berard
|
| Street address |
28 rue Laennec, 69373 Lyon cedex 08
|
| City |
Lyon |
| State/province |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA153719 |
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