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Series GSE36594 Query DataSets for GSE36594
Status Public on Mar 20, 2012
Title Transcriptome analysis of medulloblastoma tumors in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary The proto-oncogene MYCN is mis-expressed in various types of human brain tumors. To clarify how developmental and regional differences influence transformation, we transduced wild-type or mutationally-stabilized murine N-mycT58A into neural stem cells (NSCs) from perinatal murine cerebellum, brain stem and forebrain. Transplantation of Nmyc WT NSCs was insufficient for tumor formation. N-mycT58A cerebellar and brain stem NSCs generated medulloblastoma/primitive neuroectodermal tumors, whereas forebrain NSCs developed diffuse glioma. Expression analyses distinguished tumors generated from these different regions, with tumors from embryonic versus postnatal cerebellar NSCs demonstrating SHH-dependence and SHH-independence, respectively. These differences were regulated in-part by the transcription factor SOX9, activated in the SHH subclass of human medulloblastoma. Our results demonstrate context-dependent transformation of NSCs in response to a common oncogenic signal.
 
Overall design 9 (total) orthotopic tumors generated from E16C, P0C, and P0F NSCs transduced with a mutation-stabilized N-Myc were compared to transduced (but not transplanted) NSCs, NSCs transduced with GFP, and cells derived from the orthotopic tumors. These were also compared to 5 normal P7 cerebella, and 32 medulloblastoma tumors from the GTML mouse model. A cell line derived from a tumor from the GTML mouse model is also included in the comparison.
 
Contributor(s) Swartling FJ, Savov V, Persson AI, Chen J, Hackett CS, Northcott PA, Grimmer MR, Lau J, Chesler L, Perry A, Phillips JJ, Taylor MD, Weiss WA
Citation(s) 22624711
Submission date Mar 18, 2012
Last update date Mar 06, 2018
Contact name William A. Weiss
Organization name University of California, San Francisco
Department Neurology
Lab William A. Weiss
Street address 1450 3rd Street, HD-220
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (1)
GPL6096 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]
Samples (56)
GSM897247 Normal P7 Cerebellum, Biological Rep 1
GSM897248 Normal P7 Cerebellum, Biological Rep 2
GSM897249 Normal P7 Cerebellum, Biological Rep 3
Relations
BioProject PRJNA153769

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36594_RAW.tar 1.3 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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