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| Status |
Public on Mar 23, 2013 |
| Title |
Genome wide analysis of pancreatic cancer cell gene expresison exposed to stromal pancreatic stellate cells in 3D gel matrix |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Capan1 (well differentiated pancreatic cancer cell line) was co-cultured with pancreatic stellate cell line (PS1) embedded in a 3D organotypic model and gene expression was analysed in comparison to cancer cells cultured alone without stellate cells.
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| Overall design |
Pancreatic stellate cells were embedded within a gel matrix composing collagen type I and Matrigel, and cancer cells were seeded on top. The gel was lifted on to metal grid after 24 hour and fed from below. Gels were harvested on day 10 and frozen sections obtained. The cancer cell layer on top of the gel was captured by laser microdissection for RNA extraction.
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| Contributor(s) |
Kadaba R, Kocher HM |
| Citation(s) |
23359139 |
| Submission date |
Mar 23, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Hemant Kocher |
| E-mail(s) |
h.kocher@qmul.ac.uk
|
| Organization name |
Barts and the London
|
| Street address |
Charterhouse Square
|
| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (6)
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| GSM900842 |
Capan1 exposed to stellate cell rep1 |
| GSM900843 |
Capan1 exposed to stellate cell rep2 |
| GSM900844 |
Capan1 exposed to stellate cell rep3 |
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| Relations |
| BioProject |
PRJNA156911 |
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