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| Status |
Public on Oct 05, 2012 |
| Title |
Comparative profiling of chromatin state maps and transcription factor occupancy during human fetal and adult erythropoiesis |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the comparative investigation of genome-wide chromatin state maps, transcription factor (TF) occupancy, and gene expression profiles from developing red cell precursors at two developmental stages. Contrasting the similarities and differences between fetal and adult erythropoiesis provides important insights into the erythroid gene expression programs and gene regulatory networks. Specifically, comparative analyses of human erythropoiesis identify developmental stage-specific enhancers as primary determinants of stage-specific gene expression programs. We find that master regulators, such as GATA1 and TAL1, cooperatively act within active enhancers but have little predictive value for stage-specific enhancer activity. Instead, a set of stage-specific co-regulators collaborates with master regulators and contributes to differential gene expression. We further identify and validate IRF2, IRF6, and MYB as effectors of adult-stage expression program. Thus, the combinatorial assembly of master regulators and transcriptional co-regulators at developmental stage-specific enhancers controls gene expression programs and temporal regulation of transcriptional networks in a mammalian genome.
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| |
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| Overall design |
Examination of various histone modifications and transcription factor occupancy by ChIP-seq in fetal and adult proerythroblasts.
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| |
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| Contributor(s) |
Xu J, Shao Z, Orkin SH |
| Citation(s) |
23041383 |
| Submission date |
Apr 02, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Jian Xu |
| E-mail(s) |
Jian.Xu@UTSouthwestern.edu
|
| Phone |
214-648-6125
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Children's Research Institute
|
| Street address |
6000 Harry Hines Blvd. NL12.138B
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75235 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL14761 |
Helicos HeliScope (Homo sapiens) |
|
| Samples (37)
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| This SubSeries is part of SuperSeries: |
| GSE36994 |
Comparative profiling of human fetal and adult erythropoiesis |
|
| Relations |
| BioProject |
PRJNA157469 |
| SRA |
SRP012028 |
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