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| Status |
Public on Oct 15, 2014 |
| Title |
MiR-21 function in T-lymphocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
T-lymphocyte activation is efficiently mimicked in vitro by treatment with anti CD3 / anti CD28 antibodies. We report miR-21 induction upon CD3/CD28 stimulation of primary T-lymphocytes. In order to assess the function of miR-21 in T-lymphocytes we interfered with miR-21 function by lentiviral transduction of a miR-21 sponge construct. MRNA profile of miR-21 sponge and control transduced T-lymphocytes 48hrs after stimulation.
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| Overall design |
mRNA microarray profile of T-lymphocytes tranduced with either a miR-21 sponge or control construct. 3 biological replicas have been performed.
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| Contributor(s) |
Carissimi C, Carucci N, Colombo T, Piconese S, Azzalin G, Cipolletta E, Citarella F, Barnaba V, Macino G, Fulci V |
| Citation(s) |
25304039 |
| Submission date |
Apr 12, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Teresa Colombo |
| Organization name |
Consiglio Nazionale delle Ricerche (CNR)
|
| Department |
Institute of Molecular Biology and Pathology (IBPM)
|
| Street address |
p.le A. Moro 7
|
| City |
Rome |
| ZIP/Postal code |
00185 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA158939 |
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