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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 09, 2012 |
| Title |
Genome wide mapping of histone acetyltransferase MOF binding in murine ES cells (sequencing) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the application of illumina sequencing technology for high-throughput profiling of histone acetyltransferase Mof in mouse embryonic stem cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse embryonic stem cells. We find that Mof widely binds to mouse genome and mark genes that are expressed, poised for expression, or stably repressed. Mof binds to both gene promoters and coding regions, exhibiting two modes of binding. This study provides a framework for understanding the function of Mof in regulating ESC core transcription network.
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| Overall design |
Examination of Mof binding patterns in ES cells
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| Contributor(s) |
Li X, Li L, Pandey R, Byun J, Gardner K, Qin Z, Dou Y |
| Citation(s) |
22862943, 24837678 |
| Submission date |
Apr 13, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Yali Dou |
| Organization name |
University of Michigan
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| Department |
Department of Pathology, Department of Biological Chemistry
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| Lab |
Dou
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| Street address |
Rm 5215A, MS I, 1301 Catherine Rd,
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA159161 |
| SRA |
SRP012212 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE37268_RAW.tar |
3.5 Gb |
(http)(custom) |
TAR (of TXT) |
| GSE37268_mof3.out.hpeak.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| GSE37268_mof3.out.wig.gz |
1.8 Mb |
(ftp)(http) |
WIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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