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| Status |
Public on Feb 13, 2014 |
| Title |
Individual-nucleotide resolution CLIP and high-throughput sequencing to map hnRNP L-RNA interactions in HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Individual-nucleotide resolution UV-crosslinking and immunoprecipitation (iCLIP) combined with high-throughput sequencing was used to generate a transcriptome-wide binding map of hnRNP L.
Supplementary file GSE37560_hnRNPL_crosslink_site.bed includes filtered crosslink sites of hnRNPL: combining data from all 3 experiments.
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| |
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| Overall design |
3 biological replicates of hnRNP L-specific and control (Flag) co-immunoprecipitated RNA after UV-crosslinking in HeLa cells
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| |
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| Contributor(s) |
Rossbach O, Hung L, Bindereif A |
| Citation(s) |
24526010 |
| Submission date |
Apr 24, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Lee-Hsueh Hung |
| E-mail(s) |
Lee-Hsueh.Hung@chemie.bio.uni-giessen.de
|
| Phone |
0049-641-9935421
|
| Organization name |
University of Giessen
|
| Department |
Biochemistry
|
| Street address |
Heinrich-Buff-Ring 58
|
| City |
Giessen |
| State/province |
Hessen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (6)
|
| GSM921620 |
iCLIP experiment with hnRNPL antibody, biological repl. 1 |
| GSM921621 |
iCLIP experiment with hnRNPL antibody, biological repl. 2 |
| GSM921622 |
iCLIP experiment with hnRNPL antibody, biological repl. 3 |
| GSM921623 |
iCLIP experiment with FLAG antibody, biological repl. 1 |
| GSM921624 |
iCLIP experiment with FLAG antibody, biological repl. 2 |
| GSM921625 |
iCLIP experiment with FLAG antibody, biological repl. 3 |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| SRA |
SRP012488 |
| BioProject |
PRJNA162423 |
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