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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 04, 2012 |
| Title |
MiR-27a-3p in peripheral blood mononuclear cells as a potential marker for pancreatic cancer screening |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Background & Aims: MicroRNAs have been shown to offer great potential in the diagnosis of cancer. We aimed to identify microRNAs in peripheral blood mononuclear cells (PBMCs) for diagnosing pancreatic cancer (PC). Methods: PBMCs microRNA expression was investigated in three independent cohorts including 352 participants (healthy, benign pancreatic/peripancreatic diseases (BPD), and PC). First, we used sequencing technology to identify differentially expressed microRNAs in 60 PBMCs samples for diagnosing PC. Quantitative reverse-transcriptase polymerase chain reaction assay was then applied to evaluate the expression of selected microRNAs. A logistic regression model was constructed using an independent cohort. Area under the receiver operating characteristic curve (AUC) was used to evaluate diagnostic accuracy. Results: We found that PBMCs miR-27a-3p could efficiently discriminate PC from BPD (AUC=0.840; 95% CI, 0.787 to 0.885; sensitivity=82.2%, specificity=76.7%). A panel composed of PBMCs miR-27a-3p and serum CA19-9 provided a high diagnostic accuracy in differentiating PC from BPD in the clinical setting (AUC=0.886; 95% CI, 0.837 to 0.923; sensitivity=85.3%, specificity=81.6%). The satisfactory diagnostic performance of the panel persisted regardless of disease status (AUCs for tumour-node-metastasis stagesⅠ,Ⅱ, and Ⅲ were 0.881, 0.884, and 0.893, respectively). Conclusion: PBMCs miR-27a-3p could be a potential marker for PC screening. A panel composed of PBMCs miR-27a-3p and serum CA19-9 has considerable clinical value in diagnosing early-stage PC. Therefore, patients who would have otherwise missed the curative treatment window can benefit from optimal therapy.
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| Overall design |
Examination of different MicroRNA profiles in 3 types of PBMCs samples
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| Contributor(s) |
Wang W, Liu L, Li G, Chen Y, Li C, Jin D, Wang X |
| Citation(s) |
23430754 |
| Submission date |
May 02, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
xiaolin wang |
| E-mail(s) |
wwsdj2002@163.com
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| Organization name |
Zhongshan Hospital, Fudan University
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| Street address |
180 Feng Lin Rd
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| SRA |
SRP012608 |
| BioProject |
PRJNA163233 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE37710_BPD-Healthy.express.txt.gz |
15.5 Kb |
(ftp)(http) |
TXT |
| GSE37710_BPD-PDAC.express.txt.gz |
14.9 Kb |
(ftp)(http) |
TXT |
| GSE37710_PDAC-Healthy.express.txt.gz |
15.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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