|
| Status |
Public on Dec 01, 2012 |
| Title |
Direct cell conversion from human dermal fibroblast to adipocyte |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To investigate the effect of ZNF395 on adipogenesis, we tested whether ZNF395 enhance cell conversion from human dermal Fibroblast (FIB) to Adipocyte (ADP). PPARG2 was reported as a master regulator and can induce adipogenesis in non-adipogenic fibroblasts. We transduced PPARG2 with or without ZNF395 in FIB with lentivirus. Interestingly, co-transduction of PPARG2 and ZNF395 showed higher occurrence of adipocyte-like cells as compared with PPARG2 alone. Moreover, genes related with lipid metabolic process and lipid transport was significantly up-regulated in combination of PPARG2 and ZNF395. These results suggest that ZNF395 co-ordinate the transcriptional regulatory pathway with PPARG2, necessary for the induction of adipogenesis.
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| |
|
| Overall design |
Total RNA was obteined from human dermal fibroblast transduced with mock lentivirus vector (FIB_ctrl), PPARG2 (FIB_PPARG2) and co-transduced with PPARG2 and ZNF395 (FIB_PPARG2+ZNF395).
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| |
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| Contributor(s) |
Ryota H, Ogawa A, Yoshihide H, Suzuki H, Shin JW |
| Citation missing |
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| Submission date |
May 11, 2012 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Ryota Hasegawa |
| E-mail(s) |
r-hase@gsc.riken.jp
|
| Organization name |
RIKEN Yokohama
|
| Department |
Omics science center
|
| Street address |
1-7-22 Suehiro-cho Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
|
| Samples (9)
|
| GSM930089 |
Cell_conversion_Fibroblast_ctrl_rep1 |
| GSM930090 |
Cell_conversion_Fibroblast_ctrl_rep2 |
| GSM930091 |
Cell_conversion_Fibroblast_ctrl_rep3 |
| GSM930092 |
Cell_conversion_Fibroblast_PPARG2_rep1 |
| GSM930093 |
Cell_conversion_Fibroblast_PPARG2_rep2 |
| GSM930094 |
Cell_conversion_Fibroblast_PPARG2_rep3 |
| GSM930095 |
Cell_conversion_Fibroblast_PPARG2+ZNF395_rep1 |
| GSM930096 |
Cell_conversion_Fibroblast_PPARG2+ZNF395_rep2 |
| GSM930097 |
Cell_conversion_Fibroblast_PPARG2+ZNF395_rep3 |
|
| Relations |
| BioProject |
PRJNA165685 |
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