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| Status |
Public on May 16, 2012 |
| Title |
Genome-wide analysis of E157D PPARγ transcriptional activity in NIH-3T3 fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The E157D PPARγ mutation presents as an atypical case of familial partial lipodystrophy and diabetes in a human cohort and has a novel molecular mechanism. The mutation is located in the DNA-binding domain, has no dominant negative activity but disrupts transcription activation after the protein has bound the DNA. The mutation causes a transcriptional activation defect on many PPARγ target genes, while some genes are induced normally. A small number of non-PPARγ target genes are induced by the E157D mutant, possibly leading to the atypical features in this cohort.
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| Overall design |
mRNA was amplified from total RNA collected from NIH-3T3 cells expressing either wild-type or E157D PPARγ through lentiviral infection. Cells were treated with DMSO or 20µM rosiglitazone for 24 hours prior to RNA isolation in three independent experiments.
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| Contributor(s) |
Campeau PM, Astapova O, Martins R, Bergeron J, Couture P, Hegele RA, Leff T, Gagné C |
| Citation(s) |
22750678 |
| Submission date |
May 15, 2012 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Philippe M Campeau |
| E-mail(s) |
pcampeau@hotmail.com, philippe.campeau@mail.mcgill.ca
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Human Genetics
|
| Lab |
Brendan Lee Lab
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA167030 |
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