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Series GSE38132 Query DataSets for GSE38132
Status Public on Oct 03, 2012
Title ZR-75-1 microarray expression data
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Testing the hormonal response of ZR-75-1 cells to estrogen, androgens, and a combination of both homones, with view determining the crosstalk between the transcriptional programs mediated by these hormones in breast cancer cells, and comparison with matched ChIP sequencing data for AR and ERalpha.
Data analysis demonstrated reciprocal interference between 5α-dihydrotestosterone (DHT)- and estradiol (E2)-induced transcriptional programs. Specifically, regulation of 26% of E2 and 15% of DHT target genes was significantly affected by cotreatment with the other hormone, in the majority of cases (78-83%) antagonistically. Pathway analysis suggested that DHT co-treatment, for example, depleted E2-regulted pathways in cell survival and proliferation.
 
Overall design Total RNA was extractd from luminal-like breast cancer ZR-75-1 cells in quadruplicate after treatment for 16h with 10nM of E2, DHT or E2+DHT.
 
Contributor(s) Buchanan G, Need EF
Citation(s) 23023562
Submission date May 22, 2012
Last update date Feb 18, 2019
Contact name Grant Buchanan
E-mail(s) grant.buchanan@adelaide.edu.au
Organization name Basil Hetzel Institute for Translational Health Research, University of Adelaide
Department Surgery
Lab Cancer Biology Group
Street address 28 Woodville Road
City Woodville South
State/province South Australia
ZIP/Postal code 5011
Country Australia
 
Platforms (1)
GPL6884 Illumina HumanWG-6 v3.0 expression beadchip
Samples (36)
GSM935236 estradiol + medroxyprogesterone acetate E2+MPA replicate 1
GSM935237 estradiol + 5a-dihydrotestosterone E2+DHT replicate 4
GSM935238 estradiol + progesterone E2s+PROG replicate 2
Relations
BioProject PRJNA169394

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38132_RAW.tar 6.3 Mb (http)(custom) TAR
GSE38132_non-normalized.txt.gz 7.3 Mb (ftp)(http) TXT
Processed data included within Sample table
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