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| Status |
Public on Apr 29, 2024 |
| Title |
Genome-wide mapping of H3K27me3 in Daxx-depleted cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We discovered Daxx can negatively regulated H3K27me3 level via facilitating long non-coding RNA HOTAIR degradation. To further explore the biological consequence and search for affected genes, we used high throughput sequencing technology to demonstrate the enrichment of H3K27me3 on the human genome and target genes repression after depletion of Daxx. This study revealed a novel role of Daxx in regulating histone modifications via modulates long non-coding RNA stability.
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| |
|
| Overall design |
Examination of H3K27me3 distribution in Daxx-depleted and untreated HEK293T cells
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| |
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| Contributor(s) |
Huang T, Huang Y, Shih H |
| Citation missing |
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| Submission date |
May 23, 2012 |
| Last update date |
Apr 29, 2024 |
| Contact name |
Hsiu-Ming Shih |
| E-mail(s) |
hmshih@ibms.sinica.edu.tw
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Biomedical Sciences
|
| Street address |
128 Sec. 2, Academia Rd. Nankang,
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| SRA |
SRP013351 |
| BioProject |
PRJNA167336 |
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