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| Status |
Public on Jun 07, 2012 |
| Title |
A Validated Gene Signature from Peripheral Blood Mononuclear Cells in Pulmonary Hypertension Associated with Sickle Cell Disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Pulmonary hypertension (PH) is a serious complication of sickle cell disease (SCD) associated with increased mortality. Gene expression profiles of peripheral blood mononuclear cells (PBMC) have been studied in pulmonary arterial hypertension and in SCD. We hypothesized that a PBMC-derived gene signature in SCD patients may be utilized as a PH biomarker. Twenty-seven patients with homozygous SCD underwent transthoracic echocardiography and PBMC isolation. PH was defined as estimated right ventricular systolic pressure (RVSP)>30 mmHg with a peak tricuspid regurgitation velocity (TRV)>2.5m/s. Genome-wide gene expression profiles were correlated against PH severity using RVSP and TRV as surrogates, which yielded 631 potentially dysregulated transcripts.
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| Overall design |
Total RNA was isolated from PBMCs using standard molecular biology protocols without DNA contamination or RNA degradation. Sample processing (e.g., cDNA generation, fragmentation, end labeling, hybridization to Affymetrix GeneChip Human Exon 1.0 ST arrays) was performed per manufacturer’s instructions. A total of 27 African descent American patients with sickle cell disease were included in the microarray analysis.
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| Contributor(s) |
Desai AA, Ahmad H, Zhou T, Trevino S, Wade M, Raghavachari N, Peters-Lawrence M, Kato GJ, Thiruvoipati T, Turner K, Artz N, Huang Y, Patel AR, Jason YX, Lang RM, Garcia JG, Machado RF |
| Citation(s) |
22679008, 27636371 |
| Submission date |
Jun 06, 2012 |
| Last update date |
Jun 27, 2019 |
| Contact name |
Tong Zhou |
| Organization name |
University of Nevada School of Medicine
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| Street address |
1664 N Virginia St
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| City |
Reno |
| State/province |
NV |
| ZIP/Postal code |
89557 |
| Country |
USA |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA168166 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38528_RAW.tar |
596.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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