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| Status |
Public on Jun 06, 2013 |
| Title |
Gene expression data in over expressed Human Fetal Lung Fibroblasts with IPF downregulated miRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Idiopathic pulmonary fibrosis (IPF) is an untreatable fibrotic lung disease characterized by fibroblast proliferation and epithelial mesenchymal transition.miRNA let-7d and mir30b were found to be signifcantly down regulated in IPF. Compared to control we over expressed these miRNAs in Human Fetal Lung Fibroblast cell line
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| Overall design |
Fetal lung tissues were collected through the University of Pittsburgh Tissue Bank through approved IRB protocol 0506140. The samples were de-identified using an honest broker system and immediately transferred to the IRB exempt protocol PRO09040459 (PI: D. Carlisle). Total RNA was labeled with Cy3 and hybridized on Agilent 8X44K gene expression array (Agilent Technologies, Santa Clara, CA). After 17 hours hybridization, arrays were washed and scanned according to the manufacturer’s protocol.
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| Contributor(s) |
Huleihel L, Kaminski N |
| Citation(s) |
24441869 |
| Submission date |
Jun 06, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Luai Huleihel |
| E-mail(s) |
luai1212@gmail.com
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| Organization name |
University of Pittsburgh
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| Department |
Regenerative medicine
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| Lab |
Dr.Badylak
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| Street address |
450 technology drive
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| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15219 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (20)
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| GSM944814 |
control, biological rep 4 |
| GSM944815 |
control, biological rep 5 |
| GSM944816 |
let-7d over expressed, biological rep 1 |
| GSM944817 |
let-7d over expressed, biological rep 2 |
| GSM944818 |
let-7d over expressed, biological rep 3 |
| GSM944819 |
let-7d over expressed, biological rep 4 |
| GSM944820 |
let-7d over expressed, biological rep 5 |
| GSM944821 |
mir30b over expressed, biological rep 1 |
| GSM944822 |
mir30b over expressed, biological rep 2 |
| GSM944823 |
mir30b over expressed, biological rep 3 |
| GSM944824 |
mir30b over expressed, biological rep 4 |
| GSM944825 |
mir30b over expressed, biological rep 5 |
| GSM944826 |
mir30b and let-7d, biological rep 1 |
| GSM944827 |
mir30b and let-7d, biological rep 2 |
| GSM944828 |
mir30b and let-7d, biological rep 3 |
| GSM944829 |
mir30b and let-7d, biological rep 4 |
| GSM944830 |
mir30b and let-7d, biological rep 5 |
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| Relations |
| BioProject |
PRJNA168108 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38530_RAW.tar |
43.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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