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| Status |
Public on Dec 01, 2013 |
| Title |
The human AlkB homologue 4 interacts with proteins associated with transcription (expression) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The Fe(II)- and 2-oxoglutarate (2OG)-dependent dioxygenase AlkB from E. coli is a demethylase which repairs alkyl lesions in DNA, as well as RNA, through a direct reversal mechanism. Humans possess nine AlkB homologues (ALKBH1-8 and FTO). ALKBH2 and ALKBH3 display demethylase activities corresponding to that of AlkB, and both ALKBH8 and FTO are RNA modification enzymes. The biochemical functions of the rest of the homologues are still unknown. To increase our knowledge on the functions of ALKBH4 and ALKBH7 we have here performed yeast two-hybrid screens to identify interaction partners of the two proteins. While no high-confidence hits were detected in the case of ALKBH7, several proteins associated with chromatin and/or involved in transcription were found to interact with ALKBH4. For all interaction partners, the regions mediating binding to ALKBH4 comprised domains previously reported to be involved in interaction with DNA or chromatin. Furthermore, some of these partners showed nuclear co-localization with ALKBH4. However, the global gene expression pattern was only marginally altered upon ALKBH4 over-expression, and larger effects were observed in the case of ALKBH7. Although the molecular function of both proteins remains to be revealed, our findings suggest a role for ALKBH4 in regulation of gene expression or chromatin state and support the previous association of ALKBH7 with spermatogenesis.
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| Overall design |
Gene expression profiling of ALKBH4 and ALKBH7 over-expression
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| Contributor(s) |
Bjornstad LG, Meza TJ, Otterlei M, Olafsrud SM, Meza-Zepeda LA, Falnes PO |
| Citation missing |
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| Submission date |
Jul 05, 2012 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Leonardo A. Meza-Zepeda |
| E-mail(s) |
leonardm@rr-research.no
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| Organization name |
The Norwegian Radium Hospital
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| Department |
Dept. of Tumor Biology
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| Street address |
Ullernchausseen 70
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| City |
Oslo |
| ZIP/Postal code |
N-0310 |
| Country |
Norway |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (12)
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| GSM956481 |
HEK293 ALKBH4 uninduced replicate 1, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956482 |
HEK293 ALKBH4 uninduced replicate 2, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956483 |
HEK293 ALKBH4 uninduced replicate 3, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956484 |
HEK293 ALKBH4 induced replicate 1, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956485 |
HEK293 ALKBH4 induced replicate 2, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956486 |
HEK293 ALKBH4 induced replicate 3, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956487 |
HEK293 ALKBH7 uninduced replicate 1, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956488 |
HEK293 ALKBH7 uninduced replicate 2, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956489 |
HEK293 ALKBH7 uninduced replicate 3, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956490 |
HEK293 ALKBH7 induced replicate 1, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956491 |
HEK293 ALKBH7 induced replicate 2, Illumina HumanWG-6 Expression BeadChip version 3 |
| GSM956492 |
HEK293 ALKBH7 induced replicate 3, Illumina HumanWG-6 Expression BeadChip version 3 |
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| This SubSeries is part of SuperSeries: |
| GSE39135 |
The human AlkB homologue 4 interacts with proteins associated with transcription |
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| Relations |
| BioProject |
PRJNA170065 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39127_RAW.tar |
6.3 Mb |
(http)(custom) |
TAR |
| GSE39127_non-normalized2.txt.gz |
2.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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