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Status |
Public on Jul 09, 2012 |
Title |
TAL1 complex in human T-cell acute lymphoblastic leukemia (RPMI-8402) |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The oncogenic transcription factor TAL1/SCL is aberrantly overexpressed in over 40% of cases of T-cell acute lymphoblastic leukemia (T-ALL), emphasizing the importance of the TAL1-regulated transcriptional program in the molecular pathogenesis of T-ALL. Here we identify regions occupied by TAL1 and its regulatory partners HEB, E2A, and GATA3 in a T-ALL cell line (RPMI-8402).
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Overall design |
Human T-ALL cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total TAL1 (Santa Cruz SC-12984), TCF12/HEB (Santa Cruz SC-357),TCF3/E2A (Santa Cruz SC-349X), and GATA3 (Santa Cruz SC-22206).
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Contributor(s) |
Lawton LN, Young RA |
Citation missing |
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Submission date |
Jul 08, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Richard A Young |
E-mail(s) |
young_computation@wi.mit.edu
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Phone |
617-258-5219
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Organization name |
Whitehead Institute for Biomedical Research
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Lab |
Young Lab
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Street address |
9 Cambridge Center
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City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (10)
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Relations |
BioProject |
PRJNA170151 |
SRA |
SRP014150 |