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| Status |
Public on Aug 09, 2012 |
| Title |
Transcriptional profiles of sorted CD8+ and CD4+ memory T cells from CP and NP and unvaccinated control RM 7 days prior to, and 4 and 14 days after wt SIVmac239 challenge by microarray analysis. |
| Platform organism |
Homo sapiens |
| Sample organism |
Macaca mulatta |
| Experiment type |
Expression profiling by array
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| Summary |
Live-attenuated SIV vaccines (LAVs) remain the most efficacious of all vaccines in nonhuman primate models of HIV/AIDS, yet the basis of their robust protection remains poorly understood. Here, we demonstrate that the degree of LAV-mediated protection against intravenous (IV) wildtype (wt) SIVmac239 challenge strongly correlates with the magnitude and function of SIV-specific, effector-differentiated T cells in lymph node (LN), but not with such T cell responses in blood or with other cellular, humoral and innate immune parameters. Maintenance of protective T cell responses was associated with persistent LAV replication in LN, which occurred almost exclusively in follicular helper T cells. Thus, effective LAVs maintain lymphoid tissue-based, effector-differentiated, SIV-specific T cells that intercept and suppress early wt SIV amplification and, if present in sufficient frequencies, can completely control and perhaps clear infection -- an observation that provides rationale for development of safe, persistent vectors that can elicit and maintain such responses.
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| Overall design |
There are two protection outcome groups, complete protect (CP) and non-protect (NP) animals. Memory T cells (CD4+ or CD8+) were sorted for three time points, seven days pre-challenge, four days post-challenge and fourteen days post-challenge.
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| Contributor(s) |
Fukazawa Y, Park H, Cameron MJ, Lefebvre F, Lum R, Coombes N, Mahyari E, Hagen S, Bae JY, Delos Reyes M, Swanson T, Legasse AW, Sylwester A, Hansen SG, Smith AT, Stafova P, Shoemaker R, Li Y, Oswald K, Axthelm MK, McDermott A, Ferrari G, Montefiori DC, Edlefsen PT, Piatak M, Lifson JD, Sékaly RP, Picker LJ, Wilkinson PA |
| Citation(s) |
22961108 |
| Submission date |
Aug 08, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Peter A Wilkinson |
| Organization name |
Case Western Reserve University
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| Department |
Pathology / Systems Biology & Bioinformatics
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| Street address |
2103 Cornell Road
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| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44120 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (80)
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| This SubSeries is part of SuperSeries: |
| GSE40006 |
Lymph node T cell responses predict the efficacy of live attenuated SIV vaccines |
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| Relations |
| BioProject |
PRJNA172176 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39968_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE39968_non-normalized_sorts.txt.gz |
4.2 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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