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| Status |
Public on Aug 10, 2012 |
| Title |
Gene expression analysis of B-ALL cells treated with BET inhibitor |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Two human acute lymphoblastic leukemia cell lines were treated with a BET bromodomain inhibitor that blocks BET association with chromatin. These cell lines, MHH-CALL4 and MUTZ-5, each carry translocation of the CRLF2 gene into the IgH locus, and their growth was found to be susceptible to BET inhibition. Gene expression changes were analyzed in each cell line versus vehicle control.
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| Overall design |
Cell were treated for 8 hours with either DMSO or 500nM of the BET bromodomain inhbitor JQ1. Three samples for each treatment were taken and analyzed for each cell line. CEL files were analyzed at both the core and extended gene level by RMA with the Affymetrix Expression Console software.
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| Contributor(s) |
Ott CJ, Bradner JE |
| Citation(s) |
22904298 |
| Submission date |
Aug 08, 2012 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Christopher J Ott |
| E-mail(s) |
christopher_ott@dfci.harvard.edu
|
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA172218 |
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