|
| Status |
Public on Mar 06, 2013 |
| Title |
RNAseq analysis of the human neutrophil transcriptome, with and without in vitro cytokine stimulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report cytokine specific changes in gene expression in the human neutrophil transcriptome using TNF-alpha and GM-CSF stimulation of healthy neutrophils
|
| |
|
| Overall design |
Healthy human neutrophils were stimulated with TNF-alpha or GM-CSF for 1h in vitro. RNA was analysed by SOLiD and Illumina sequencing. RNA from one biological donor was sequenced on both platforms, and two different biological donors were sequenced by Illumina.
|
| |
|
| Contributor(s) |
Wright HL, Thomas HB, Moots RJ, Edwards SW |
| Citation(s) |
23554905, 27042300 |
| Submission date |
Aug 31, 2012 |
| Last update date |
Jul 03, 2019 |
| Contact name |
Helen Louise Wright |
| E-mail(s) |
hlwright@liverpool.ac.uk
|
| Organization name |
University of Liverpool
|
| Department |
Institute of Life Course and Medical Sciences
|
| Street address |
6 West Derby Street
|
| City |
Liverpool |
| ZIP/Postal code |
L7 8TX |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13393 |
AB SOLiD 4 System (Homo sapiens) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA174350 |
| SRA |
SRP015360 |
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