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| Status |
Public on Mar 22, 2013 |
| Title |
Derivation of small molecule Human Neural Precursor Cells (smNPCs) from human pluripotent stem cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We have developed a method for derivation of smNPCs from human embryonic (hESC) and induced pluripotent stem cells (hIPSC), that are only dependent on small molecules stimulating WNT and SHH signalling for their propagation.
These smNPC cultures were enzymatically split every five to six days.
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| Overall design |
Human pluripotent stem cells were differentiated into the neural lineage using double SMAD inhibition, together with stimulation of WNT and SHH pathways. This led to expansion of neural epithelium, which was further cultured for 20 passages.
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| Citation(s) |
23533608 |
| Submission date |
Sep 03, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Peter Reinhardt |
| E-mail(s) |
peter.reinhardt@mpi-muenster.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Street address |
Von-Esmarch-Straße 54
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA174291 |
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